superresolution microscope carl  (Carl Zeiss)

Journal: The Journal of Biological Chemistry

Article Title: Glycans function as a Golgi export signal to promote the constitutive exocytic trafficking

doi: 10.1074/jbc.RA120.014476

Figure Lengend Snippet: Tac-TC accumulates at the interior of the trans -Golgi cisternae; HeLa cells were used. A , schematic diagram showing the domain organization of Tac and Tac-TC RUSH reporters. The panel i s organized as described for A . ss-Streptavidin-KDEL is the ER hook. Strep ., streptavidin. The 5 Thr residues that are potentially under mucin-type O -glycosylation are indicated on Tstem. B and C , intra-Golgi transport kinetics of Tac and Tac-TC as revealed by the LQ versus time plot. Cells transiently co-expressing the RUSH reporter, SBP-GFP-Tac or SBP-GFP-Tac-TC, and GalT-mCherry were treated with nocodazole to induce the formation of Golgi mini-stacks. The ER-arrested RUSH reporter was subsequently released by the administration of biotin. At different time points, cells were immunostained for endogenous GM130, and the LQs for the RUSH reporter were acquired and fitted to the first-order exponential function. Error bars , mean ± S.E.; n , number of Golgi mini-stacks quantified. Adj. R 2 , adjusted R 2 . D–F , Tac-TC localizes to the interior of Golgi cisternae at the steady state. D , Airyscan superresolution images of the en face and side view of giantin and GFP-Tac-TC in the Golgi mini-stack. Cells transiently expressing GFP-Tac-TC were treated with nocodazole to induce the formation of Golgi mini-stacks and stained for endogenous giantin. The en face and side view images were selected. Scale bar , 500 nm. The en face averaging of giantin and GFP-Tac-TC in the Golgi mini-stack is shown in E . The corresponding radial mean intensity profile is displayed in F , with the distance from the center of fluorescence mass (giantin peak is normalized to 100) as the x axis and the radial mean intensity (normalized) as the y axis. n , number of Golgi mini-stack images used.

Article Snippet: This was conducted using Airyscan superresolution microscope system (Carl Zeiss) as described previously ( ).

Techniques: Glycoproteomics, Expressing, Staining, Fluorescence

Journal: Applied and Environmental Microbiology

Article Title: Magnetotactic Bacteria Accumulate a Large Pool of Iron Distinct from Their Magnetite Crystals

doi: 10.1128/AEM.01278-20

Figure Lengend Snippet: Red (left panels), green (center panels), and merged (right panels) fluorescence images obtained from superresolution microscopy of wild-type, Δ mamP , and Δ mamT AMB-1 incubated with FIP-1 for 180 min. The two Δ mamP panels show the two fluorescence patterns (diffuse and located to the magnetosome chains, as in wild-type) observed in the populations. Scale bars = 4 μm. Additional pictures are available in the supplemental material.

Article Snippet: All bacterial samples were observed by structured illumination microscopy with a Carl Zeiss Elyra PS.1 superresolution fluorescence microscope, using red (excitation wavelength of 561 nm, emission wavelength of 570 to 620 nm) and green (excitation wavelength of 488 nm, emission wavelength of 495 to 550 nm) laser lines for the detection of the native and cleaved probe, respectively.

Techniques: Fluorescence, Microscopy, Incubation